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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白CD109 antigen, Cd109含量
- 适应物种:
小鼠
- 标记物:
CD109 antigen, Cd109
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse CD109 antigen, Cd109 ELISA试剂盒
Product Name: Mouse CD109 antigen, Cd109 ELISA试剂盒
Product Name: Mouse CD109 antigen, Cd109 ELISA试剂盒
Gene Name: Mouse CD109 antigen, Cd109
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse CD109 antigen, Cd109
Alternative Name: Mouse CD109 antigen, Cd109
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse CD109 antigen, Cd109 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse CD109 antigen, Cd109 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse CD109 antigen, Cd109 ELISA试剂盒 has been pre-coated with an Mouse CD109 antigen, Cd109 antibody specific to Mouse CD109 antigen, Cd109 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse CD109 antigen, Cd109 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse CD109 antigen, Cd109 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse CD109 antigen, Cd109 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验thousands of colored products. Thus, in ELISA, antigens are detectable when present in only nanogram and picogram quantities. One form of the ELISA is carried out in a microtiter plate which is used as the solid phase for adsorption of antigen (Figure
Antigen Design Sera Purification Tech Sheet
will briefly explore peptide selection and design, coupling strategy, and carrier proteins which are important factors in anti-peptide antisera generation. Serum purification will also be discussed. For more complete coverage of antigen design, please refer
as antigen. 2. In a 96-well ELISA plate (Nunc MaxiSorp is best), add 100 ng of antigen in 50 µL in each well you will be using for your test, as well as four control wells. The perimeter wells on the plate are generally not used, as they tend to give poor
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