- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-04927m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Protein FAM132B, Fam132b含量
- 适应物种：
小鼠
- 标记物：
Protein FAM132B, Fam132b
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Protein FAM132B, Fam132b ELISA试剂盒
Product Name: Mouse Protein FAM132B, Fam132b ELISA试剂盒
Product Name: Mouse Protein FAM132B, Fam132b ELISA试剂盒
Gene Name: Mouse Protein FAM132B, Fam132b
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Protein FAM132B, Fam132b
Alternative Name: Mouse Protein FAM132B, Fam132b
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Protein FAM132B, Fam132b ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Protein FAM132B, Fam132b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Protein FAM132B, Fam132b ELISA试剂盒 has been pre-coated with an Mouse Protein FAM132B, Fam132b antibody specific to Mouse Protein FAM132B, Fam132b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Protein FAM132B, Fam132b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Protein FAM132B, Fam132b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Protein FAM132B, Fam132b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
反应发生的风险，故而在合成磷酸化多肽时，需要对氨基酸投料量、反应方法以及反应时长等进行优化调整以期达到更理想、更经济的合成效果。我们有针对性地对磷酸化多肽合成条件进行了探索和调整，采用最终的优化条件成功合成了含有多达六个磷酸化丝氨酸残基的多肽：FAM-Ahx-X(pS)XX(pS)X(pS)X(pS)XX(pS)X(pS)-NH2（客户肽，详细序列未给出；其氨基端标记FAM以进行荧光检测），经过RP-HPLC纯化后最终纯品的纯度高达95%（见Figure 3）。 参考文献： 1. P
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones. Procedure 1. All incubations
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