- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-04679m
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Sphingosine 1-phosphate receptor 1, S1pr1含量
- 适应物种：
小鼠
- 标记物：
Sphingosine 1-phosphate receptor 1, S1pr1
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Sphingosine 1- phosphate receptor 1, S1PR1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Sphingosine 1-phosphate receptor 1(S1P receptor 1,S1P1),;Sphingosine 1-phosphate receptor Edg-1(S1P receptor Edg-1),Endothelial differentiation G-protein coupled receptor 1,Lysophospholipid receptor B1,
Search name
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Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse sphingosine 1-phosphate receptor 1,S1P receptor 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to S1P receptor 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for S1P receptor 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain S1P receptor 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of S1P receptor 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Studying Wound Repair in the Mouse
in a reproducible, controlled environment. Here, we detail methods for full?thickness excisional and incisional wounding and for analysis of wound biopsies. Curr. Protoc. Mouse Biol . 3:171?185 © 2013 by John Wiley & Sons, Inc. Keywords: wound
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
. Activation of the lung S1P1 receptor reduces allergen‐induced plasma leakage in mouse. Brit. J. Pharmacol. 158:1295‐1301. Delayre‐Orthez, C., Becker, J., De Blay, F., Frossard, N
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