- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-04587m
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Transcription factor 4, Tcf4含量
- 适应物种：
小鼠
- 标记物：
Transcription factor 4, Tcf4
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Transcription factor 4, Tcf4 ELISA试剂盒
Product Name: Mouse Transcription factor 4, Tcf4 ELISA试剂盒
Product Name: Mouse Transcription factor 4, Tcf4 ELISA试剂盒
Gene Name: Mouse Transcription factor 4, Tcf4
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Transcription factor 4, Tcf4
Alternative Name: Mouse Transcription factor 4, Tcf4
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Transcription factor 4, Tcf4 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Transcription factor 4, Tcf4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Transcription factor 4, Tcf4 ELISA试剂盒 has been pre-coated with an Mouse Transcription factor 4, Tcf4 antibody specific to Mouse Transcription factor 4, Tcf4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Transcription factor 4, Tcf4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Transcription factor 4, Tcf4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Transcription factor 4, Tcf4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验wywdxfsh 在ncbi上查不到这个咚咚，只有转录因子 transcription factor 4 我做的小鼠的 freecell T细胞因子(TCF)/淋巴增强因子(LEF)是Wnt信号传导通路的主要信号分子。TCF/LEF家族包括LEF-1、TCF-1、 TCF-3、TCF-4。其中TCF-4是Wnt信号传导通路的一个重要的分子，并且在肿瘤的发生过程中起重要作用. SEE: http
PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues
for the parallel analysis of the activities of more than 200 transcription factor proteins, using an advanced oligonucleotide array-based transcription factor assay (OATFA) platform, to assay TF activities in mice. The system uses a PCR-based system to translate
浅析染色质免疫沉淀（ChIP）技术在 DNA 与蛋白质相互作用研究中的重要性
-19]； xChIP：用来研究DNA及低结合力蛋白，采用甲醛或紫外线进行 DNA 和蛋白交联，超声波片段化染色质，然后进行片段富集及后续分析，适用于多数非组蛋白的蛋白，例如 [9, 15, 20]。 X-ChIP 试验的一般过程 以上两种方法在分离 DNA-蛋白质复合物之后，对 DNA 进行 PCR 扩增，验证目标序列的存在。除验证实验外，ChIP DNA也可以进行测序分析，这种方法被称为 ChIP-seq [22] ；也可做芯片分析，这种方法被称为 ChIP on CHIP 或 ChIP
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