- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-04528m
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Adenosine deaminase, Ada含量
- 适应物种：
小鼠
- 标记物：
Adenosine deaminase, Ada
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Adenosine deaminase, Ada ELISA试剂盒
Product Name: Mouse Adenosine deaminase, Ada ELISA试剂盒
Product Name: Mouse Adenosine deaminase, Ada ELISA试剂盒
Gene Name: Mouse Adenosine deaminase, Ada
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Adenosine deaminase, Ada
Alternative Name: Mouse Adenosine deaminase, Ada
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Adenosine deaminase, Ada ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Adenosine deaminase, Ada concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Adenosine deaminase, Ada ELISA试剂盒 has been pre-coated with an Mouse Adenosine deaminase, Ada antibody specific to Mouse Adenosine deaminase, Ada .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Adenosine deaminase, Ada and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Adenosine deaminase, Ada , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Adenosine deaminase, Ada in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验免疫的联合缺陷，对各种病原生物都易感，临床上常发生反复肺部感染、口腔念珠菌感染、慢性腹泻、败血症等。在治疗方面可选用正常骨髓干细胞移植或同胞兄妹骨髓移植。但供体骨髓中T细胞介导的移埴物抗宿主反应（GVH）往往是造成治疗失败的严重问题。 约有25％～50％的重症联合免疫缺陷病例，主要与先天性缺乏腺苷脱氨酶（adenosine deaminase，ADA）有关。一则严重影响细胞DNA包括淋巴细胞DNA的合成代谢，同时由于三磷酸脱氧腺苷在淋巴细胞的堆积，后者对淋巴细胞尤其是T细胞具有一定的毒性
和核苷酸水平上分别由腺苷脱氨酶(adenosine deaminase,ADA)和腺苷酸脱氨酸(AMP deaminase)催化脱氨生成次黄嘌呤核苷或次黄嘌呤核苷酸。它们再水解成次黄嘌呤，并在黄嘌呤氧化酶(xanthine oxidase)的催化下逐步氧化为黄嘌呤和尿酸(uric acid)。ADA的遗传性缺乏，可选择性清除淋巴细胞，导致严重联合免疫缺陷病(Severe combined immunodeficiency olisease,SCID)。 体内嘌呤核苷酸的分解
【交流】复旦学子报道高剂量siRNA在小鼠中可以抑制RNAi
of siRNA, and the rebound was associated with an increase in the mRNA level of meri-1 (mouse enhanced RNAi) and adar-1 (adenosine deaminase acting on RNA) genes encoding an exonuclease and RNA-specific adenosine deaminase respectively. Down-regulation
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