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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Serine-protein kinase ATM, Atm含量
- 适应物种:
小鼠
- 标记物:
Serine-protein kinase ATM, Atm
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Serine-protein kinase ATM, Atm ELISA试剂盒
Product Name: Mouse Serine-protein kinase ATM, Atm ELISA试剂盒
Product Name: Mouse Serine-protein kinase ATM, Atm ELISA试剂盒
Gene Name: Mouse Serine-protein kinase ATM, Atm
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Serine-protein kinase ATM, Atm
Alternative Name: Mouse Serine-protein kinase ATM, Atm
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Serine-protein kinase ATM, Atm ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Serine-protein kinase ATM, Atm concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Serine-protein kinase ATM, Atm ELISA试剂盒 has been pre-coated with an Mouse Serine-protein kinase ATM, Atm antibody specific to Mouse Serine-protein kinase ATM, Atm .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Serine-protein kinase ATM, Atm and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Serine-protein kinase ATM, Atm , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Serine-protein kinase ATM, Atm in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Identifying Inhibitors of ATM and ATR Kinase Activities
family of serine/threonine-directed kinases that contain C-terminal kinase domains bearing significant sequence homology to those of mammalian and yeast phosphoinositide 3-kinases (1 ). In response to genotoxic insults, these kinases phosphorylate
Analyzing the Regulation and Function of ATM
transfected cell line. ATM protein induction is monitored by immunoblotting with antibodies against both ATM and a FLAG sequence tag in the recombinant protein. Extracts from irradiated cells are immunoprecipitated with anti-ATM antibodies, and protein kinase
Detecting ATM-Dependent Chromatin Modification in DNA Damage and Heat Shock Response
The ataxia telangiectasia-mutated gene product (ATM), whose loss of function is responsible for ataxia telangiectasia (A-T), is a protein kinase that interacts with several substrates and is implicated in mitogenic signal transduction
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