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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Adam17含量
- 适应物种:
小鼠
- 标记物:
Adam17
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Adam17 ELISA试剂盒
Product Name: Mouse Adam17 ELISA试剂盒
Product Name: Mouse Adam17 ELISA试剂盒
Gene Name: Mouse Adam17
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Adam17
Alternative Name: Mouse Adam17
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Adam17 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Adam17 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Adam17 ELISA试剂盒 has been pre-coated with an Mouse Adam17 antibody specific to Mouse Adam17 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Adam17 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Adam17 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Adam17 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 ADAM17(TACE)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 ADAM17 单抗包被于酶标板上,标准品和样品中的 ADAM17 与单抗结合,加入生物素化的抗人 ADAM17 ,形成免疫复合物连接在板上,辣根
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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