Mouse Proto- oncogene c- Fos, FOS ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
c-Fos,proto-oncogene c-Fos; cellular oncogene c-fos; AP-1; p55; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); G0/G1 switch regulatory protein 7; activator protein 1; FBJ murine osteosarcoma viral oncogene homolog,Proto-oncogene c-Fos,Cellular oncogene fos,G0/G1 switch regulatory protein 7,
Search name
Mouse c-Fos ELISA KIT ,Mouse CFOS ELISA KIT ,Mouse proto-oncogene c-Fos ELISA KIT ,Mouse cellular oncogene c-fos ELISA KIT ,Mouse AP-1 ELISA KIT ,Mouse p55 ELISA KIT ,Mouse FBJ murine osteosarcoma viral (v-fos) oncogene homolog ELISA KIT ,Mouse oncogene FOS ELISA KIT ,Mouse G0/G1 switch regulatory protein 7 ELISA KIT ,Mouse activator protein 1 ELISA KIT ,Mouse FBJ murine osteosarcoma viral oncogene homolog ELISA KIT ,Mouse Proto-oncogene c-Fos ELISA KIT ,Mouse Cellular oncogene fos G0/G1 switch regulatory protein 7 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Proto-oncogene c-Fos concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proto-oncogene c-Fos. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Proto-oncogene c-Fos and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Proto-oncogene c-Fos, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Proto-oncogene c-Fos in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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