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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Pancreatic prohormone, Ppy含量
- 适应物种:
小鼠
- 标记物:
Pancreatic prohormone, Ppy
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Pancreatic prohormone, Ppy ELISA试剂盒
Product Name: Mouse Pancreatic prohormone, Ppy ELISA试剂盒
Product Name: Mouse Pancreatic prohormone, Ppy ELISA试剂盒
Gene Name: Mouse Pancreatic prohormone, Ppy
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Pancreatic prohormone, Ppy
Alternative Name: Mouse Pancreatic prohormone, Ppy
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Pancreatic prohormone, Ppy ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Pancreatic prohormone, Ppy concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Pancreatic prohormone, Ppy ELISA试剂盒 has been pre-coated with an Mouse Pancreatic prohormone, Ppy antibody specific to Mouse Pancreatic prohormone, Ppy .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pancreatic prohormone, Ppy and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pancreatic prohormone, Ppy , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pancreatic prohormone, Ppy in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Assessment of Insulin Secretion in the Mouse
Insulin is synthesized by the β cells of the pancreatic islets as part of a single 110-amino acid precursor, preproinsulin ( see Fig. 1 ). Processing is initiated by removal of the amino terminal, 24-amino acid signal
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
Support Protocol 3: Mouse Euthanasia and Tissue Dissection Support Protocol 4: Serum Insulin Quantification Using ELISA Support Protocol 5: Analysis of Human Cell Engraftment by Flow Cytometry
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