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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白DNA topoisomerase 2-beta, Top2b含量
- 适应物种:
小鼠
- 标记物:
DNA topoisomerase 2-beta, Top2b
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse DNA topoisomerase 2-beta, Top2b ELISA试剂盒
Product Name: Mouse DNA topoisomerase 2-beta, Top2b ELISA试剂盒
Product Name: Mouse DNA topoisomerase 2-beta, Top2b ELISA试剂盒
Gene Name: Mouse DNA topoisomerase 2-beta, Top2b
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse DNA topoisomerase 2-beta, Top2b
Alternative Name: Mouse DNA topoisomerase 2-beta, Top2b
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse DNA topoisomerase 2-beta, Top2b ELISA试剂盒 allows for the in vitro quantitative determination of Mouse DNA topoisomerase 2-beta, Top2b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse DNA topoisomerase 2-beta, Top2b ELISA试剂盒 has been pre-coated with an Mouse DNA topoisomerase 2-beta, Top2b antibody specific to Mouse DNA topoisomerase 2-beta, Top2b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse DNA topoisomerase 2-beta, Top2b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse DNA topoisomerase 2-beta, Top2b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse DNA topoisomerase 2-beta, Top2b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase IV Activities
DNA gyrase and DNA topoisomerase (topo) IV are the bacterial targets of coumarin and quinolone antimicrobial agents. Widespread resistance to clinically important antibiotics such as beta-lactams and macrolides has stimulated the development
from Mouse Liver (Chastain) Very basic protocol for students Genomic DNA Isolation from Blood (Roe Lab) Standard protocol for DNA isolation from blood Microdissection and DNA Extraction from Paraffin
Transgenic Mouse and Transgenic Rat Outline
an endogenous mouse gene, such as beta-globin, or an endogenous rat gene, such as prolactin, is required in order to demonstrate that the DNA preparations are free of PCR inhibitors. Animals are tested with both assays so that no transgenic founder is mistakenly
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