- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-02322m
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Interleukin-7, Il7含量
- 适应物种：
小鼠
- 标记物：
Interleukin-7, Il7
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Interleukin-7, Il7 ELISA试剂盒
Product Name: Mouse Interleukin-7, Il7 ELISA试剂盒
Product Name: Mouse Interleukin-7, Il7 ELISA试剂盒
Gene Name: Mouse Interleukin-7, Il7
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Interleukin-7, Il7
Alternative Name: Mouse Interleukin-7, Il7
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Interleukin-7, Il7 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Interleukin-7, Il7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Interleukin-7, Il7 ELISA试剂盒 has been pre-coated with an Mouse Interleukin-7, Il7 antibody specific to Mouse Interleukin-7, Il7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Interleukin-7, Il7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Interleukin-7, Il7 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Interleukin-7, Il7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验ELISAs and Interleukin Research
ELISA (enzyme-linked immunosorbent assay) is a powerful, versatile, precise, and reliable quantitative technique for the measurement of antigens or antibodies in biologic samples. The ELISA technique is a widely used tool in biologic
Measurement of Interleukin-2 by Bioassay
immunosorbent assay (ELISA) methods, they are relatively easy to set up in a cell culture laboratory.
预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定人血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL7 含量。需自备的设备及试剂1.450±10nm 滤光片的酶标仪 （建议仪器使用前提前预热）2. 单道或多道微量加液器及吸头3. 稀释样品的 EP 管4. 蒸馏水或去离子水5. 吸水纸6. 盛放洗液的容器标本的采集与保存1. 血清：将收集于血清分离管的全血标本在室温放置 2 小时或 4oC 过夜，然后 1000×g 离心 20 分钟，取上清即可，或将上清置于 - 20
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