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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Amiloride-sensitive amine oxidase [copper-containing], A含量
- 适应物种:
小鼠
- 标记物:
Amiloride-sensitive amine oxidase [copper-containing], A
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Amiloride- sensitive amine oxidase [copper- containing], ABP1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Amiloride-sensitive amine oxidase [copper-containing],DAO,Diamine oxidase,Kidney amine oxidase,KAO,Amiloride-binding protein,ABP,Histaminase, DAO,AOC1,ABP; ABP1; DAO1; KAO; amiloride binding protein 1 amine oxidase (copper-containing); amiloride-binding protein 1; amiloride-sensitive amine oxidase; amiloride-sensitive amine oxidase [copper-containing]; amine oxidase copper domain-containing protein 1; diamine oxidase; histaminase; kidney amine oxidase; amine oxidase copper containing 1
Search name
Mouse Amiloride-sensitive amine oxidase [copper-containing] ELISA KIT ,Mouse DAO ELISA KIT ,Mouse Diamine oxidase ELISA KIT ,Mouse Kidney amine oxidase ELISA KIT ,Mouse KAO ELISA KIT ,Mouse Amiloride-binding protein ELISA KIT ,Mouse ABP ELISA KIT ,Mouse Histaminase ELISA KIT ,Mouse DAO ELISA KIT ,Mouse AOC1 ELISA KIT ,Mouse ABP ELISA KIT ,Mouse ABP1 ELISA KIT ,Mouse DAO1 ELISA KIT ,Mouse KAO ELISA KIT ,Mouse amiloride binding protein 1 amine oxidase (copper-containing) ELISA KIT ,Mouse amiloride-binding protein 1 ELISA KIT ,Mouse amiloride-sensitive amine oxidase ELISA KIT ,Mouse amiloride-sensitive amine oxidase [copper-containing] ELISA KIT ,Mouse amine oxidase copper domain-containing protein 1 ELISA KIT ,Mouse diamine oxidase ELISA KIT ,Mouse histaminase ELISA KIT ,Mouse kidney amine oxidase ELISA KIT ,Mouse amine oxidase copper containing 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse amiloride-sensitive amine oxidase [copper-containing],DAO concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DAO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DAO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DAO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DAO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验(Component B), that have no reactivity. Using the two kit components with any aminereactive dye will provide distinct positive and negative populations of beads. You can use the AbC™ Anti-Mouse Bead Kit and the ArC™ Amine Reactive Compensation Bead Kit
8 for that tube. 10) Resuspend the bead pellet by adding 0.5 mL of buffer to each sample tube. 11) Add one drop of negative beads (Component B in the AbC™ Anti-Mouse Bead Kit) to sample tube(s) containing the AbC™ capture beads. 12) Add
Methods for the Detection of D-Amino-Acid Oxidase
work, my colleagues and I concluded that D-amino-acid oxidase is not present in the mouse liver (13 ). This paper examines in greater detail the methods used in reaching that conclusion. 4 ). Organs, tissues, or tissue culture cells
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