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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Complement factor H, Cfh含量
- 适应物种:
小鼠
- 标记物:
Complement factor H, Cfh
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Complement factor H, Cfh ELISA试剂盒
Product Name: Mouse Complement factor H, Cfh ELISA试剂盒
Product Name: Mouse Complement factor H, Cfh ELISA试剂盒
Gene Name: Mouse Complement factor H, Cfh
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Complement factor H, Cfh
Alternative Name: Mouse Complement factor H, Cfh
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Complement factor H, Cfh ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Complement factor H, Cfh concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Complement factor H, Cfh ELISA试剂盒 has been pre-coated with an Mouse Complement factor H, Cfh antibody specific to Mouse Complement factor H, Cfh .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Complement factor H, Cfh and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Complement factor H, Cfh , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Complement factor H, Cfh in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Purification, Quantification, and Functional Analysis of Complement Factor H
Complement Factor H (FH) is an abundant, non-enzymic plasma/serum glycoprotein, which has a major role in regulating activation of the complement system. It can be purified from human plasma/serum by affinity chromatography, using
Anti-Factor H Autoantibodies Assay
of autoantibodies directed against Factor H (FH), the main plasmatic alternative complement pathway regulatory protein. Their presence induces an acquired functional FH deficiency. The anti-FH autoantibodies screening must be performed at the very onset
Factor H-related proteins (CFHRs) are plasma glycoproteins related in structure and antigenicity to each other and to the complement inhibitory protein factor H. Such proteins are found in most mammals but their number and domain composition
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