BH3-interacting domain death a
gonist, Bid ELISA KIT/ Mouse BH3-interacting domain death agonist, Bid ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-02238m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白BH3-interacting domain death agonist, Bid含量
- 适应物种：
小鼠
- 标记物：
BH3-interacting domain death agonist, Bid
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse BH3-interacting domain death agonist, Bid ELISA试剂盒
Product Name: Mouse BH3-interacting domain death agonist, Bid ELISA试剂盒
Product Name: Mouse BH3-interacting domain death agonist, Bid ELISA试剂盒
Gene Name: Mouse BH3-interacting domain death agonist, Bid
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse BH3-interacting domain death agonist, Bid
Alternative Name: Mouse BH3-interacting domain death agonist, Bid
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse BH3-interacting domain death agonist, Bid ELISA试剂盒 allows for the in vitro quantitative determination of Mouse BH3-interacting domain death agonist, Bid concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse BH3-interacting domain death agonist, Bid ELISA试剂盒 has been pre-coated with an Mouse BH3-interacting domain death agonist, Bid antibody specific to Mouse BH3-interacting domain death agonist, Bid .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse BH3-interacting domain death agonist, Bid and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse BH3-interacting domain death agonist, Bid , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse BH3-interacting domain death agonist, Bid in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验相关专题 细胞凋亡专题 线粒体 凋亡途径是细胞凋亡的主要途径之一，是目前研究凋亡的热点.各种凋亡刺激信号通过BH3(Bcl-2 homology domain 3)-only蛋白引起Bax(Bcl-2-asslciated protein X)蛋白移位到线粒体外膜并多聚化,形成膜通道,刺激线粒体释放细胞色素C(Cyt C) 和Smac(second mitochondrial-derived activator
. In many cases, information from the environment is relayed to a central 'apoptosis rheostat' at the mitochondrion by stimulus-specific induction, modification or movement of pro-apoptotic 'BH3 domain-only' members of the Bcl-2 family (including
，我们使用神经保护剂BI-6C9，BI-6C9是一种公认的BH-3 小分子抑制剂，可与死亡激动剂（domain death agonist，BID）发生相互作用，是 Bcl-2基因家族的预凋亡成员（4，5）。 总之，研究表明，xCELLigence系统可通过多方面实验，持续对神经细胞培养进行监测，所进行的实验包括接种反应板、预培养、增殖胶质细胞的去除、药物作用，以及对神经毒性与神经保护作用的确认。 材料与方法 HT-22类神经元细胞 按照指定密度接种 HT-22细胞
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