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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Selenoprotein P, SEP含量
- 适应物种:
小鼠
- 标记物:
Selenoprotein P, SEP
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Selenoprotein P, SEP ELISA试剂盒
Product Name: Mouse Selenoprotein P, SEP ELISA试剂盒
Product Name: Mouse Selenoprotein P, SEP ELISA试剂盒
Gene Name: Mouse Selenoprotein P, SEP
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Selenoprotein P, SEP
Alternative Name: Mouse Selenoprotein P, SEP
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Selenoprotein P, SEP ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Selenoprotein P, SEP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Selenoprotein P, SEP ELISA试剂盒 has been pre-coated with an Mouse Selenoprotein P, SEP antibody specific to Mouse Selenoprotein P, SEP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Selenoprotein P, SEP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Selenoprotein P, SEP , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Selenoprotein P, SEP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验to a goat antimouse IgG, which catalyzes the substrate p-nitrophenyl phosphate, to form a colored product. Excess reagents are removed by washing, and enzyme activity can be measured with an ELISA plate reader at 415nm. The amount of colored product
of antigen or antibody concentration. There are two main variations on this method: The ELISA can be used to detect the presence of antigens that are recognized by an antibody or it can be used to test for antibodies that recognize an antigen. An ELISA
E L I S A w i t h p l a t e l e t s
OUTLINE This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) (shown here). PROTOCOL
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