Mouse ANG- (1- 7) ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Angiotensin (1-7),ang(1-7), ANG1-7, Angiotensin1-7
Search name
Mouse Angiotensin (1-7) ELISA KIT ,Mouse ang(1-7) ELISA KIT ,Mouse ANG1-7 ELISA KIT ,Mouse Angiotensin1-7 ELISA KIT, Mouse ANG- (1- 7) ELISA Kit
Intended use
Mouse ANG- (1- 7) ELISA Kit allows for the in vitro quantitative determination of Mouse Angiotensin (1-7) concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Mouse ANG- (1- 7) ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin (1-7), During the reaction, Angiotensin (1-7) in the sample or standard competes with a fixed amount of biotin-labeled Angiotensin (1-7) for sites on a pre-coated Monoclonal antibody specific to Angiotensin (1-7). Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Angiotensin (1-7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse ANG- (1- 7) ELISA Kit Range 0.156-10ng/mL
Mouse ANG- (1- 7) ELISA Kit PDF Manual download
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