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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Octamer Binding Transcription Factor 4含量
- 适应物种:
小鼠
- 标记物:
Octamer Binding Transcription Factor 4
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Octamer Binding Transcription Factor 4 ELISA试剂盒
Product Name: Mouse Octamer Binding Transcription Factor 4 ELISA试剂盒
Product Name: Mouse Octamer Binding Transcription Factor 4 ELISA试剂盒
Gene Name: Mouse Octamer Binding Transcription Factor 4
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Octamer Binding Transcription Factor 4
Alternative Name: Mouse Octamer Binding Transcription Factor 4
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Octamer Binding Transcription Factor 4 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Octamer Binding Transcription Factor 4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Octamer Binding Transcription Factor 4 ELISA试剂盒 has been pre-coated with an Mouse Octamer Binding Transcription Factor 4 antibody specific to Mouse Octamer Binding Transcription Factor 4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Octamer Binding Transcription Factor 4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Octamer Binding Transcription Factor 4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Octamer Binding Transcription Factor 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues
for the parallel analysis of the activities of more than 200 transcription factor proteins, using an advanced oligonucleotide array-based transcription factor assay (OATFA) platform, to assay TF activities in mice. The system uses a PCR-based system to translate
) ( 2 - 4 ). The proximal promoter, containing the hormone response elements (HREs), and binding sites for nuclear factor 1 (NF1), TATA binding protein (TBP), and octamer transcription factors (OTFs) is encompassed by nucleosomes A and B ( 2 , 5 ). Analysis
Mobility Shift DNA‐Binding Assay Using Gel Electrophoresis
., and Sharp, P.A. 1986. A single polypeptide possesses the binding and activities of the adenovirus major late transcription factor. Mol. Cell. Biol. 6:4723‐4733. Fried, M
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