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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白cyclic citrullinated peptide含量
- 适应物种:
小鼠
- 标记物:
cyclic citrullinated peptide
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse cyclic citrullinated peptide ELISA试剂盒
Product Name: Mouse cyclic citrullinated peptide ELISA试剂盒
Product Name: Mouse cyclic citrullinated peptide ELISA试剂盒
Gene Name: Mouse cyclic citrullinated peptide
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse cyclic citrullinated peptide
Alternative Name: Mouse cyclic citrullinated peptide
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse cyclic citrullinated peptide ELISA试剂盒 allows for the in vitro quantitative determination of Mouse cyclic citrullinated peptide concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse cyclic citrullinated peptide ELISA试剂盒 has been pre-coated with an Mouse cyclic citrullinated peptide antibody specific to Mouse cyclic citrullinated peptide .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse cyclic citrullinated peptide and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse cyclic citrullinated peptide , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse cyclic citrullinated peptide in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries
without the need for any special equipment, and therefore can be employed in any chemical or biochemical laboratory. OBOC peptide libraries have been widely used to map the ligand specificity of proteins, to determine the substrate specificity of enzymes, and to develop inhibitors
in four different osteoblastic cell lines and in isolated neonatal mouse calvarial bone cells has been examined. In the rat osteosarcoma cell line UMR-106-01, vasoactive intestinal polypeptide (VIP, 0.001-1 microM), calcitonin gene-related peptide (CGRP, 0.3-30 nM
根据临床症状和x线表现诊断,从而导致误诊和漏诊,因此人们一直在寻找特异、敏感的实验室指标以助诊断。 2000年Schellekens等以合成的环瓜氨酸肽(cyclic citrullinated peptide,CCP)作为底物用ELISA方法检测RA血清中的抗体,发现此抗体对RA具有很高的特异性和敏感性,可用于RA的诊断。抗CCP抗体的检测为RA的早期诊断提供了较好的血清学指标,目前国内正在逐渐开展临床应用。 ◆抗环瓜氨酸肽抗体的生物学特性 抗环瓜氨酸肽
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