- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELA-E1132r
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Rat InterferonAlphaAntibody含量
- 适应物种：
小鼠
- 标记物：
Rat InterferonAlphaAntibody
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Rat InterferonAlphaAntibody ELISA试剂盒
Product Name: Rat InterferonAlphaAntibody ELISA试剂盒
Product Name: Rat InterferonAlphaAntibody ELISA试剂盒
Gene Name: Rat InterferonAlphaAntibody
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Rat InterferonAlphaAntibody
Alternative Name: Rat InterferonAlphaAntibody
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Rat InterferonAlphaAntibody ELISA试剂盒 allows for the in vitro quantitative determination of Rat InterferonAlphaAntibody concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Rat InterferonAlphaAntibody ELISA试剂盒 has been pre-coated with an Rat InterferonAlphaAntibody antibody specific to Rat InterferonAlphaAntibody .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rat InterferonAlphaAntibody and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat InterferonAlphaAntibody , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rat InterferonAlphaAntibody in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
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