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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白corticotropin releasing hormone含量
- 适应物种:
小鼠
- 标记物:
corticotropin releasing hormone
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse corticotropin releasing hormone ELISA试剂盒
Product Name: Mouse corticotropin releasing hormone ELISA试剂盒
Product Name: Mouse corticotropin releasing hormone ELISA试剂盒
Gene Name: Mouse corticotropin releasing hormone
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse corticotropin releasing hormone
Alternative Name: Mouse corticotropin releasing hormone
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse corticotropin releasing hormone ELISA试剂盒 allows for the in vitro quantitative determination of Mouse corticotropin releasing hormone concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse corticotropin releasing hormone ELISA试剂盒 has been pre-coated with an Mouse corticotropin releasing hormone antibody specific to Mouse corticotropin releasing hormone .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse corticotropin releasing hormone and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse corticotropin releasing hormone , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse corticotropin releasing hormone in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Characterization of Corticotropin‐Releasing Factor (CRF) Receptors
., and Chrousos, G.P. 1996. In vivo and in vitro characterization of antalarmin, a nonpeptide corticotropin‐releasing hormone (CRH) receptor antagonist: Suppression of pituitary ACTH release and peripheral inflammation. Endocrinology. 137:5747‐5750
Mammalian adaptation to stressful stimuli requires activation of the type 1 corticotropin releasing hormone (CRH) receptor (CRH-R1), a 415 aminoacid protein that belongs to the large superfamily of 7 transmembrane domain receptors that relay
Functional Identification of Peptide Receptors
significance. Even though precise physiological functions could be assigned to insulin, parathyroid hormone (PTH), calcitonin, growth hormone (GH), and growth hormone releasing factor (GHRH), corticotropin (ACTH) and corticotropin releasing factor (CRF), atria
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