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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白 B-Cell Activating Factor含量
- 适应物种:
小鼠
- 标记物:
B-Cell Activating Factor
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse B-Cell Activating Factor ELISA试剂盒
Product Name: Mouse B-Cell Activating Factor ELISA试剂盒
Product Name: Mouse B-Cell Activating Factor ELISA试剂盒
Gene Name: Mouse B-Cell Activating Factor
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse B-Cell Activating Factor
Alternative Name: Mouse B-Cell Activating Factor
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse B-Cell Activating Factor ELISA试剂盒 allows for the in vitro quantitative determination of Mouse B-Cell Activating Factor concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse B-Cell Activating Factor ELISA试剂盒 has been pre-coated with an Mouse B-Cell Activating Factor antibody specific to Mouse B-Cell Activating Factor .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse B-Cell Activating Factor and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse B-Cell Activating Factor , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse B-Cell Activating Factor in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factors
factor-κB ligand (RANKL). The identification and significance of any one of these myeloma-derived OAFs is dependent on robust and reliable assays that measure the de novo formation and activation of OCs. A number of in vitro assay systems
and virus-infected cells is the release of perforin and granzyme proteins. Perforin proteins form pores in the membranes of the attacked cell, allowing the entry of Granzyme A and Granzyme B. Granzyme B induces caspase activation and cleavage of factors
. to 1 liter. 4. 30% H2O2 5. OPD Stock, 4.0% 4 g OPD in 100 ml di-H2O. Aliquot and store at -20oC. Protect from light. 6. 4.5N H2SO4 12.0 ml Concentrated Sulfuric Acid 88.0 ml di-H2O 7. Hanks Balanced Salt Solution (HBSS) B. Procedure 1. Wash ELISA
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