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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Amylase Beta含量
- 适应物种:
小鼠
- 标记物:
Amylase Beta
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Amylase Beta ELISA试剂盒
Product Name: Mouse Amylase Beta ELISA试剂盒
Product Name: Mouse Amylase Beta ELISA试剂盒
Gene Name: Mouse Amylase Beta
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Amylase Beta
Alternative Name: Mouse Amylase Beta
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Amylase Beta ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Amylase Beta concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Amylase Beta ELISA试剂盒 has been pre-coated with an Mouse Amylase Beta antibody specific to Mouse Amylase Beta .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Amylase Beta and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Amylase Beta , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Amylase Beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠淀粉酶 (Amylase)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 Amylase 单抗包被于酶标板上,标准品和样品中的 Amylase 与单抗结合,加入生物素化的抗大鼠 Amylase
FA Extraction of [insoluble] Amyloid beta from Mouse Brain
each sample individually for one minute on ice. Immerse the tip of the probe in the sample, then move tube up and down while sonicating. 3. Spin 400 μl at 135,000 x g for 1 hr at 4°C (50,000 rpm in a TLA 100.3 rotor). 4. Dilute 210 μl
【求助】关于TGF-Beta,FN酶联免疫(ELISA)试剂盒的选择参与者:txl4fox我是要酶联免疫(ELISA)的方法测细胞上清液中的TGF-Beta,FN抗原的表达情况,关于ELISA试剂盒的选择我想请教几个问题:我想买酶标板自己包被一抗,那我应该如何选择ELISA试剂盒?有没有不带酶标板只带一抗,二抗,酶底物,以及底物显色剂而且够做五六张板的ELISA试剂盒?如果没有,是不是我应该分开订购?ELISA试剂盒从国外哪几个公司订购比较好同时又相对不会很贵?谢谢。参与者:风来了你说的市场
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