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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Fatty acid binding protein 4 含量
- 适应物种:
小鼠
- 标记物:
Fatty acid binding protein 4
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Fatty acid binding protein 4 ELISA Kit
Product Name:Mouse Fatty acid binding protein 4 ELISA Kit
Packing:96T
Catalog No.:ELA-E0184m
Gene Name:Mouse FABP4
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse FABP4
Alternative Name:Mouse Fatty acid binding protein 4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Fatty acid binding protein 4 ELISA Kit allows for the in vitro quantitative determination of Mouse FABP4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Fatty acid binding protein 4 ELISA Kit has been pre-coated with an Mouse Fatty acid binding protein 4 antibody specific to Mouse FABP4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse FABP4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse FABP4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse FABP4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验the concentration of unbound FA is a method using the fluorescent-(acrylodan) labeled intestinal FA-binding protein (ADIFAB). Under appropriate conditions, ADIFAB fluorescence measures unbound FA, regardless of any third phase such as albumin, FA-binding proteins
Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes
The authors’ laboratory has applied a series of different fluorescence assays for monitoring the binding and transport of fatty acids (FA) in model and biological membranes. The authors recently expanded their fluorescent assays
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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