hydroxyproline, Hyp ELISA Kit/

Mouse hydroxyproline, Hyp ELISA试剂盒

Product Name: Mouse hydroxyproline, Hyp ELISA试剂盒

Product Name: Mouse hydroxyproline, Hyp ELISA试剂盒

Gene Name: Mouse hydroxyproline, Hyp

Detect Range:78-5000pg/ml

Sensitivity:39pg/ml

Target Protein Name: Mouse hydroxyproline, Hyp

Alternative Name: Mouse hydroxyproline, Hyp
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description: Mouse hydroxyproline, Hyp ELISA试剂盒 allows for the in vitro quantitative determination of Mouse hydroxyproline, Hyp concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse hydroxyproline, Hyp ELISA试剂盒 has been pre-coated with an Mouse hydroxyproline, Hyp antibody specific to Mouse hydroxyproline, Hyp .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse hydroxyproline, Hyp and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse hydroxyproline, Hyp , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse hydroxyproline, Hyp in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!