- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- CELI-66003m
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Phosphatidylethanolamine, PE含量
- 适应物种：
小鼠
- 标记物：
Phosphatidylethanolamine, PE
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Phosphatidylethanolamine, PE ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Phosphatidylethanolamine,;PE
Search name
Mouse PE ELISA Kit, Mouse Phosphatidylethanolamine ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Phosphatidylethanolamine concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the c ompetitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Phosphatidylethanolamine, During the reaction, Phosphatidylethanolamine
in the sample or
standard competes with a fixed amount of biotin-labeled Phosphatidylethanolamine for sites on a pre-coated Monoclonal antibody
specific to Phosphatidylethanolamine . Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Phosphatidylethanolamine in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
：
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
. Cell Sorting (Lin-,cKit+,Sca1+): 1. Incubate cells in FcgRII block on ice for 10 min. 2. Stain an aliquot and with PE-IgG2a and FITC-IgG2b isotype antibodies as neg. controls. Incubate remainder of cells with Sca-1 PE, plus c-kit FITC antibodies
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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