The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using SNAI1 antibody.
Immunohistochemistry of paraffin-embedded rat kidney using SNAI1 antibody.
Immunohistochemistry of paraffin-embedded human liver using SNAI1 antibody.
Immunohistochemistry of paraffin-embedded mouse brain using SNAI1 antibody.
Background:
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3