Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human BAX
Calculated molecular weight
4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa
Observed molecular weight
23kDa
Swiss Prot
Q07812
Gene ID (NCBI)
581
Alternative Names
BAX; BCL2L4; apoptosis regulator BAX
Application Notes:
WB
1:500 - 1:1000
IHC
1:50 - 1:100
IF
1:50 - 1:100
Western blot analysis of extracts of various cell lines, using BAX antibody.
Immunofluorescence analysis of C6 cells using BAX antibody.
Immunofluorescence analysis of NIH/3T3 cells using BAX antibody.
Immunofluorescence analysis of U2OS cells using BAX antibody.
Background:
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.