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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pQE-30 UA
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pQE-30 UA/ pQE-30 UA璐ㄧ矑
pQE-30 UA
PVT13414 2ug
pQE-30 UA Search name
pQE-30 UA,pQE-30 UA vector,pQE-30 UA plasmid,pQE-30 UA map
pQE-30 UA Information
Bacterial Resistance(s): Amp
Screening:
Growth Strain(s): DH5 alpha
Growth Condition: LB/37 Centigrade
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文献和实验偶然发现的一个pQE30系统表达蛋白纯化案例,帖出来和大家分享。 Purification of LF. 诱导与表达 E. coli SG13009(pREP4) carrying the recombinant plasmid pPG-LF1 was grown at 37°C in Luria broth with 100 μg of ampicillin and 25 μg of kanamycin per ml at 250 rpm. When the A600
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
This protocol gives M13 quality sequence when used with the Quick DNA Plasmid Prep. protocol D.2. 1、Solutions Denaturation Solution 2 M NaOH 200 ml 10N NaOH 2 mM EDTA 4 ml 0.5 M EDTA pH 8.0 up to 1 ml with Q store at room temperature
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