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- LSM BIO
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- PVT11125
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pLB2 CAG P2Gm
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pLB2 CAG P2Gm/ pLB2 CAG P2Gm璐ㄧ矑
pLB2 CAG P2Gm
Catalog No. PVT11125
Packing 2ug
Function /
Resistance /
Screen /
Strain /
Culture temperature
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
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文献和实验This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
% SDS and vortex for 1min Freeze samples at -20°C Thaw Vortex Start QIAGEN miniprep protocol by adding 180µl of Buffer P1 to obtain a final volume of 250µl Add 250µl Buffer P2 Etc.. follow QIAGEN protocol Elute
Plasmid Mini Purification Protocol
should then be stored at 4℃ and is stable for 6 months. •Check Buffer P2 for SDS precipitation due to low storage temperatures.If necessary, dissolve the SDS by warming to 37℃. •Pre-chill Buffer P3 at 4℃.It should be in the refrigerator, along with P1. Day
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