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- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pAnc80L65
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pAnc80L65/ pAnc80L65璐ㄧ矑
pAnc80L65
Catalog No. PVT11047
Packing 2ug
Function /
Resistance Amp
Screen /
Strain Stbl3
Culture temperature 37degrees centigrade
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
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文献和实验, and resuspend in 10 µl of TE, pH 8.0. Use 2 µl of each for analysis on a 1% agarose gel. Purification of cosmids and low-copy-number plasmids From: QIAGEN Plasmid Mini Handbook Cosmids and low-copy-number plasmids often require large culture volumes
This protocol gives M13 quality sequence when used with the Quick DNA Plasmid Prep. protocol D.2. 1、Solutions Denaturation Solution 2 M NaOH 200 ml 10N NaOH 2 mM EDTA 4 ml 0.5 M EDTA pH 8.0 up to 1 ml with Q store at room temperature
with 1 volume of chloroform x1, spin 2 min. Transfer aqueous phase to a new tube. 9) Add 1 volume of isopropanol, mix. Spin at max. speed x 2 min. 10) Rinse with 70% ethanol. Dry pellet. Resuspend in 80 µL of T.E. Optional (for DNA sequencing) 11) Add 20
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