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- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pCold TF
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pCold TF/ pCold TF璐ㄧ矑
pCold TF
Catalog No. PVT10602
Packing 2ug
Function E.coli expression plasmid
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon pBR322
Copy Low
Promoter CSPA
Induction IPTG
Forward primer pCold-F
Reverse primer pCold-R
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文献和实验Gold Nanoparticle-Enhanced Electroporation for Leukemia Cell Transfection
system with pWizGFP plasmid DNA probes. The efficiency was found to be dependent on the size, concentration, and mixing ratio of free AuNPs/Tf-AuNPs. An equivalent mixture of free AuNPs and Tf-AuNPs exhibited the best enhancement with the transfection efficiency
Functional Analysis of Transcription Factors by Microparticle Bombardment
mosaic virus (CaMV) 35S promoter. The second plasmid includes a reporter luciferase (LUC) gene transcribed from a promoter containing the TF binding site(s). The third plasmid contains a reference β-glucuronidase (GUS) gene transcribed from the 35S-CaMV
system. In the case of pMJ05, this difficulty has been alleviated by using the Sulfolobus virus SSV1 as the vector backbone. The ability of the pMJ05 plasmid to spread in the culture as a virus, the availability of an effective selection marker (pyr ) and of tunable
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