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- LSM BIO
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- PVT10338
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pcDNA Flag Lamp 2b-HA
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pcDNA Flag Lamp 2b-HA/ pcDNA Flag Lamp 2b-HA璐ㄧ矑
pcDNA Flag Lamp 2b-HA
Catalog No. PVT10338
Packing 2ug
Function ORF expression plasmid
Resistance /
Screen /
Strain /
Culture temperature
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
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文献和实验Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
Figure 19.20.1 The Strep/FLAG tandem affinity purification. (A ) N‐ and C‐terminal SF‐TAP tags (POI, protein of interest). (B ) Overview of both purification steps. (1) Purification by the tandem Strep‐tag II moiety: binding to Strep‐Tactin matrix
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
the plasmid DNA with very high fidelity. Plasmid DNA is physically independent of genomic DNA and this allows for their easy isolation from bacterial cultures. the pcDNA3.1+ plasmidVector plasmids used for cloning are short, circular, double-stranded DNA
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
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