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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pEGFP-hsp27 wt FL
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pEGFP-hsp27 wt FL/ pEGFP-hsp27 wt FL璐ㄧ矑
pEGFP-hsp27 wt FL
Catalog No. PVT10237
Packing 2ug
Function ORF expression plasmid
Resistance Kan
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
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文献和实验Enzyme Kinetics assay of the WT
* The day before transfection, split and plating the cells, so the that they are 50%- 60% confluent the day of transfection. Avoid antibiotics at the time of transfection and during. * Culture vessel:100mm * plasmid DNA ; 4 microg
Restriction Map and Multiple Cloning Site of pEGFP-N2. (Unique restriction sites are in color or bold.) The Not I site follows the EGFP stop codon. The Nhe I site cannot be used for fusions since it contains an in-frame stop codon
多克隆位点区 Restriction Map and Multiple Cloning Site of pEGFP-N1. (Unique restriction sites are in color or bold.) The Not I site follows the EGFP stop codon. The Xba I site (*) is methylated in the DNA provided by CLONTECH. If you wish to digest
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