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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pBiT1.1-N [TK-LgBiT]
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pBiT1.1-N [TK-LgBiT]
Product Name: pBiT1.1-N [TK-LgBiT]
Cat No.: PVT18339
Alternative Name: pBiT1.1-N [TK-LgBiT],pBiT1.1-N [TK-LgBiT] vector,pBiT1.1-N [TK-LgBiT] plasmid,pBiT1.1-N [TK-LgBiT] map,pBiT1.1-N [TK-LgBiT] sequence
Plasmid form: Freeze-dried powder
Storage Condition: -20 Centigrade
Bulk order
Promoter: TK
Bacterial Resistance(s): Amp
Screening: -
Growth Strain(s): DH5alpha
Culture Medium: LB
Temperature: 37 Centigrade
Vector Size:
*Product is for research use only!
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文献和实验【求助】最近在做hsv tk,所用前药为粉末状GCV(购自sigma),但不知道怎么溶解
qulinyd 最近在做hsv tk,所用前药为粉末状GCV(购自sigma),但不知道怎么溶解合适,还请各位帮帮忙哦 freecell 你从sigma买来的东西,应该有个datasheet吧: Preparation Instructions Soluble at 10 mg/ml in 0.1 N HCl. Storage/Stability Store desiccated
of bacteria o.n. in LB + 100 mg/mL ampicillin. 2) Spin down 1.5 mL of each culture (5,000 rpm x 3 min.) Discard supernatant. Add additional 1.5 mL of cell suspension and spin a second time. 3) Resuspend pellet in 100 µL of H2O. 4) Add 300 µL
pellet with 70% EtOH . 13.vacuum dry pellet for 5 min and take up in 100 ml 1x TE/20 mg/ml RNAse A . 14.usually 1-2 ml is enough for digest (high-copy plasmid), keep DNA frozen at -20℃. 15.phenolize important preps if to be kept
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