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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pGL4.15[luc2P Hygro]
- 库存:
10
- 供应商:
LSMBIO
- 规格:
质粒干粉
pGL4.15[luc2P Hygro]
Product Name: pGL4.15[luc2P Hygro]
Cat No.: PVT17664
Alternative Name: pGL4.15[luc2P Hygro],pGL4.15[luc2P Hygro] vector,pGL4.15[luc2P Hygro] plasmid,pGL4.15[luc2P Hygro] map,pGL4.15[luc2P Hygro] sequence
Plasmid form: Freeze-dried powder
Storage Condition: -20 Centigrade
Bulk order
Promoter: inquiry
Bacterial Resistance(s): Amp
Screening: inquiry
Growth Strain(s): DH5alpha
Culture Medium: LB
Temperature: 37 Centigrade
Vector Size:
*Product is for research use only!
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文献和实验Recovery plasmids from dead bacterial/plasmid
We store our all of our plasmids as bacterial host stocks at -80 stored in 7% DMSO. Such stocks are very long-lived giving robust bacterial growth even after 10-15 years. However, occasionally we have had a stock that performs poorly.
and in liquid media, respectively. Hygromycine (Hyg) was used at 30 μg/ml on plates and 15 μg/ml in liquid media for selection of the pSC101BADγβαA[hygro] vector and streptomycin (Stp) was used at a concentration of 75 μg/ml. Heat-treated chlor
Construction of Gene-Targeting Vectors by Recombineering
and made recombineering competent by pSim18 plasmid transformation. Exon 4, which encodes the main functional domains of the Bcl11a protein, is selected to be the deleted region and would be flanked by lox P sites in the conditional allele. (B ) The Bsd cassette flanked
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