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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
EGFP-Rab7A Q67L
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
EGFP-Rab7A Q67L
Product Name: EGFP-Rab7A Q67L
Cat No.: PVT17461
Alternative Name: EGFP-Rab7A Q67L,EGFP-Rab7A Q67L vector,EGFP-Rab7A Q67L plasmid,EGFP-Rab7A Q67L map,EGFP-Rab7A Q67L sequence
Plasmid form: Freeze-dried powder
Storage Condition: -20 Centigrade
Bulk order
Promoter: CMV
Bacterial Resistance(s): Amp
Screening: G420/EGFP
Growth Strain(s): DH5alpha
Culture Medium: LB
Temperature: 37 Centigrade
Vector Size:
*Product is for research use only!
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文献和实验This protocol gives very clean plasmid preps for restriction digests and cloning
This is a standard large scale prep.for plasmid DNA which gives a yield of 0.5-1.0mg.I have made some minor changes to the MHB protocol. Solutions Solution I,II,III from protocol D.1. Tris/EDTA pH 7.5 (optional) 20 ml 1 M Tris 7.5 4 ml
pH 8.0 25 ml 1M Tris pH 8.0 10 mM EDTA 20 ml 0.5 M EDTA pH 8.0 up to 1 liter with Q store at 4 ℃ Solution II 1% SDS 2.5 ml 20% SDS 0.2N NaOH 1.0 ml 10N NaOH up to 50 ml with Q make fresh as needed Solution III 25% potassium acetate 250 g
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