The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:100
IF
1:50 - 1:100
IP
1:20 - 1:50
Western blot analysis of extracts of various cell lines, using SQSTM1 antibody.
Immunohistochemistry of paraffin-embedded human esophagus using SQSTM1 antibody.
Immunofluorescence analysis of U2OS cells using SQSTM1 antibody.
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug SQSTM1 antibody.
Background:
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.