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文献和实验Application of the Firefly Luciferase Reporter Gene
) as a reporter gene proved to be both laborious and onerous. In contrast, the luciferase gene possesses intrinsic qualities that most other reporter genes lack greater sensitivity, ease of use, instantaneous quantification, “ environment friendliness
Analysis of Rhythmic Gene Expression in Adult Drosophila Using the Firefly Luciferase Reporter Gene
by the extraction of clock gene products. This procedure makes it impossible to analyze molecular rhythms in an individual over time. To measure clock gene expression within the living animal, firefly luciferase can be used as real-time reporter gene. This chapter
The Application of Bacterial Luciferase as a Reporter Gene in Mycobacteria
Bioluminescence is the product of two distinct enzymes, firefly luciferase and bacterial luciferase. The application of the firefly enzyme in the study of mycobacteria has been described by other groups (1 ,2 ) (see Chapter 31
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