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100 µL
Alkaline Phosphatase/ALPL|Alkaline Phosphatase/ALPL antibody|Alkaline Phosphatase/ALPL抗体|Anti-Human 兔单抗(产品说明)
抗体种属:Human
抗体靶点:ALPL
抗体应用:WB
抗原货号:
抗原描述:A synthetic peptide corresponding to the center region of the Human Alkaline Phosphatase / ALPL
抗体宿主:Rabbit
抗体Ig类型:Rabbit IgG
抗体纯化方法:Protein A
抗体标记:
抗体制备:This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Alkaline Phosphatase / ALPL. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
抗体特异性:Human Alkaline Phosphatase / ALPL
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
salt NBT, (Altman, 1972) can be used in conjunction with X-Phos as the final electron acceptor for the indoxyl dimerization reaction. When reduced, NBT forms a purple precipitate. Of the cloned phosphatases, the human placental alkaline
Western Blotting with Alkaline Phosp
Western Blotting with Alkaline Phosphatase ConjugatesSAMPLE PREPARATIONFor Protein Concentration Determination of Cell Culture1.Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS).2.Aspirate
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
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