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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human Alpha-fetoprotein / AFP Protein (Catalog#12177-H08H)
- 亚型:
Mouse IgG1
- 形态:
液体
- 保存条件:
在-20℃ to -80℃
- 克隆性:
MAb
- 适应物种:
鼠
- 保质期:
12个月
- 抗原来源:
12177-H08H
- 目录编号:
12177-MM26
- 级别:
免疫学试剂
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 宿主:
Mouse
- 应用范围:
ELISA(Det)
- 靶点:
AFP
- 抗体英文名:
Alpha-fetoprotein / AFP Antibody, Mouse MAb
- 抗体名:
Alpha-fetoprotein / AFP 鼠单抗
- 规格:
20 µL/100 µL
| 规格: | 20 µL | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 100 µL | 产品价格: | ¥1699.0 |
抗体种属:Human
抗体靶点:AFP
抗体应用:ELISA(Det)
抗原货号:12177-H08H
抗原描述:Recombinant Human Alpha-fetoprotein / AFP Protein (Catalog#12177-H08H)
抗体宿主:Mouse
抗体Ig类型:0
抗体纯化方法:Protein A
抗体制备:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Alpha-fetoprotein / AFP (rh Alpha-fetoprotein / AFP; Catalog#12177-H08H; P02771; Met1-Val609). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
抗体特异性:Human Alpha-fetoprotein / AFP
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验2, Wu, M; et al. Visualizing alpha-fetoprotein level in undiluted serum based on microfluidic particle accumulation. Sensors and Actuators B: Chemical
3, Jin, Y; Nan, G. Generation of induced pluripotent stem cell(iPSC)line CJUHi001-A derived peripheral blood mononuclear cells of spinocerebellar ataxia type 1(SCA1) the CAG repeat mutation in ATXN1 gene. Stem Cell Research
In situ hybridisation to alpha satellite sequences (chromosome specific)
solution and replace with mouse anti-biotin antibody diluted 1:100 in blocking solution for 40 minutes at 37C. We currently use DAKO antibody #M0743, 100-200m l per slide. (10) Wash with blocking solution for 10 minutes (11) Incubate with rabbit
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00)(3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfate for every 100 ml Sup.(5) 4L 100 mM Tris/pH
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