The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using U2AF1 antibody.
Immunohistochemistry of paraffin-embedded rat Intestine using U2AF1 antibody.
Immunohistochemistry of paraffin-embedded human oophoroma using U2AF1 antibody.
Immunohistochemistry of paraffin-embedded mouse liver using U2AF1 antibody.
Background:
This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.