LOCUS Exported 5264 bp ds-DNA circular SYN 22-10-2015
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5264)
AUTHORS admin
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5264
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 609..665
/note="MCS"
/note="multiple cloning site"
misc_feature 667..1253
/note="IRES2"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 1254..1931
/codon_start=1
/product="improved tetrameric variant of DsRed fluorescent
protein"
/note="DsRed2"
/note="mammalian codon-optimized"
/translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
LFL"
polyA_signal 2052..2173
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2180..2635)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2662..2766
/gene="bla"
/note="AmpR promoter"
promoter 2768..3125
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2976..3111
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 3160..3954
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4186..4233
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4562..5150
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
721 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
781 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
841 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
901 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
961 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
1021 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
1081 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
1141 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
1201 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggcct
1261 cctccgagaa cgtcatcacc gagttcatgc gcttcaaggt gcgcatggag ggcaccgtga
1321 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggccacaaca
1381 ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
1441 cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca
1501 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
1561 gcgtggcgac cgtgacccag gactcctccc tgcaggacgg ctgcttcatc tacaaggtga
1621 agttcatcgg cgtgaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct
1681 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagacccaca
1741 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtct atctacatgg
1801 ccaagaagcc cgtgcagctg cccggctact actacgtgga cgccaagctg gacatcacct
1861 cccacaacga ggactacacc atcgtggagc agtacgagcg caccgagggc cgccaccacc
1921 tgttcctgta gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
1981 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
2041 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
2101 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
2161 tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
2221 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
2281 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
2341 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
2401 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
2461 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
2521 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
2581 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
2641 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
2701 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg