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- PVT10165
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pCDNA3.1-HA-Tip60
- 库存：
100
- 供应商：
LSMBIO
- 规格：
2ug
pCDNA3.1-HA-Tip60
Catalog No. PVT10165
Packing 2ug
pCDNA3.1-HA-Tip60 Information
Function ORF expression plasmid
Alias: NM_182709.2, KAT5, cPLA2; ESA1; HTA
Plasmid Classification: Gene Library Vector; Human Mammalian Cell Expression Plasmid
Prokaryotic resistance: Ampicillin
Screening marker: Neomycin Neo/hereditary mycin G418
Cloning strain: Escherichia coli DH5alpha
Culture conditions: 37鈩?/span>
pCDNA3.1-HA-Tip60 Sequences
LOCUS Exported 7293 bp ds-DNA circular SYN 27-FEB-2018
FEATURES Location/Qualifiers
source 1..7293
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 202..581
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 582..785
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 830..848
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 874..900
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
misc_feature 952..2337
/label=Tip60
polyA_signal 2860..3084
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3130..3558
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3572..3901
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3752..3887
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3968..4762
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=Neo
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4936..5057
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind 5130..5146
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5154..5184)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5199..5220
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5508..6093)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6264..7124)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7125..7229)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
841 actataggga gacccaagct ggctagcgcc atgtacccat acgatgttcc agattacgct
901 cggatccaag aattaattcc cgggatatcg tcgacccacg cgtccgggaa gatggcggag
961 gtgggggaga taatcgaggg ctgccgccta cccgtgctgc ggcggaacca ggacaacgaa
1021 gatgagtggc ccctggccga gatcctgagc gtgaaggaca tcagtggccg gaagcttttc
1081 tacgtccatt acattgactt caacaaacgt ctggatgaat gggtgacgca tgagcggctg
1141 gacctaaaga agatccagtt ccccaagaaa gaggccaaga cccccactaa gaacggactt
1201 cctgggtccc gtcctggctc tccagagaga gaggtgaaac ggaaggtgga ggtggtttca
1261 ccagcaactc cagtgcccag cgagacagcc ccggcctcgg tttttcccca gaatggagcc
1321 gcccgtaggg cagtggcagc ccagccagga cggaagcgaa aatcgaattg tttgggcact
1381 gatgaggact cccaggacag ctctgatgga ataccgtcag caccacgcat gactggcagc
1441 ctggtgtctg atcgaagcca cgacgacatc gtcacccgga tgaagaacat tgagtgcatt
1501 gagctgggcc ggcaccgcct caagccgtgg tacttctccc cgtacccaca ggaactcacc
1561 acattgcctg tcctctacct gtgcgagttc tgcctcaagt acggccgtag tctcaagtgt
1621 cttcagcgtc atttgaccaa gtgtgaccta cgacatcctc caggcaatga gatttaccgc
1681 aagggcacca tctccttctt tgagattgat ggacgtaaga acaagagtta ttcccagaac
1741 ctgtgtcttt tggccaagtg tttccttgac cataagacac tgtactatga cacagaccct
1801 ttcctcttct acgtcatgac agagtatgac tgtaagggct tccacatcgt gggctacttc
1861 tccaaggaga aagaatcaac ggaagactac aatgtggcct gcatcctaac cctgcctccc
1921 taccagcgcc ggggctacgg caagctgctg atcgagttca gctatgaact ctccaaagtg
1981 gaagggaaaa cagggacccc tgagaagccc ctctcagacc ttggcctcct atcctatcga
2041 agctactggt cccagaccat cctggagatc ctgatggggc tgaagtcgga gagcggggag
2101 aggccacaga tcaccatcaa tgagattagt gaaatcacca gcatcaagaa ggaggatgtc
2161 atctccactc tgcagtacct caatctcatc aactactaca agggccagta catcctcaca
2221 ctgtcagagg acatcgtgga tggccatgag cgggccatgc tcaagcggct cctgcggatc
2281 gactccaagt gtctgcactt cactcccaag gactggagca agagggggaa gtggtgacca
2341 gacactgccc actgcagtgc caagacggca gcaggactgg ggctgatagc ccaccccgcc
2401 cccactgcag ctcccacaaa gcactctaag ggagatgggg ctgaggacag ctcaaaaagg
2461 agaggacagg cctggcaggg gcccactggt gcccagcacc aaggcgagct ccgggctcag
2521 accaactcca aggtcagctg gccacaggcc caggcctcct ctgaagcagg gaccagaggg
2581 agccaggcag ctgtgtacag tgagaaggga tccggatggg ggagctctgt acagagggct
2641 ggtgattgta aaaatttctt ttgtaaagta gaagttgggg gtggggtggg tgctggctgc
2701 aaaaatttct ggcttctctt acccctattg cccccggcaa taaattgttt ctatatgcca
2761 aaaaaaaaaa aaaaaagggc ggccaattct gcagatatcc agcacagtgg cggccgctcg
2821 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
2881 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
2941 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
3001 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
3061 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
3121 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
3181 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
3241 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
3301 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
3361 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
3421 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
3481 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
3541 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
3601 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
3661 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
3721 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
3781 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
3841 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
3901 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
3961 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
4021 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
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文献和实验Recombinant DNA Engineering, Or Cloning Genes In Plasmids
the plasmid DNA with very high fidelity. Plasmid DNA is physically independent of genomic DNA and this allows for their easy isolation from bacterial cultures. the pcDNA3.1+ plasmidVector plasmids used for cloning are short, circular, double-stranded DNA
Hi guys, I have a question for you. I made a construct which had been sequenced. It is in pcDNA3.1(+) and with HA-TAA to stop. The right size for endogenous protein is 31kD. But when I transfected into COS-7 and run western, the size
Detection of ERK1/2 Activities Using Affinity Reagents
-7 cells were transfected with pCDNA3-HA-ERK2 or pECE-HA-MAPK (HA-p38α) in six-well plates using the DEAE-dextran method (1 μg of DNA/well). Cells were activated with 50 ng/mL of EGF for 5 min (for HA-ERK2; lanes 1 and 2) or with 0.5 M sorbitol for 20 min
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