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pAN7-1 Plasmid

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    • 保存条件：

      负20摄氏度

    • 保质期：

      3个月

    • 英文名：

      pAN7-1

    • 库存：

      50

    • 供应商：

      LSMBIO

    • 规格：

      2ug

    pAN7-1

    Search name

    pAN7-1,Plasmid pAN7-1,pAN7-1 vector

    pAN7- 1 Plasmid information

    Promoter: gpdA
    Replicon: pUC ori
    Terminator: TRPC
    Plasmids: other host vectors; filamentous fungal expression plasmids
    Plasmid size: 6756bp
    Prokaryotic resistance: ampicillin Ampicillin
    Screening markers: hygromycin Hygromycin
    Cloned strain: DH5 alpha
    Culture conditions: 37 centigrade, aerobic, LB
    Expression host: filamentous fungi
    Culture conditions: Reference Literature
    Induction mode: no induction
    5'sequencing primers: Based on full sequence design
    3'sequencing primers: Based on full sequence design
    Note: NCBI: Z32698.1
    Use:Filamentous fungal plasmid

    pAN7- 1 Plasmid description

    Reference

    1閵嗕笒ffect of de-phosphorylation of linearized pAN7-1 and of addition of restriction enzyme on plasmid integration in Penicillium paxilli.
    2閵嗕箑ransformation of sapstaining fungi with hygromycin B resistance plasmids pAN7-1 and pCB1004.
    3閵嗕赋adro de integrao de pAN7-1 em mutantes de Magnaporthe grisea com patogenicidade alterada em arroz.
    4閵嗕浮ntegra??o de pAN7-1 no Genoma de Magnaporthe grisea Mediada por Enzima de Restri??o.
    5閵嗕赋rimary structure of the trpC gene from nidulans.
    6閵嗕箑ransformation of based on the hygromycin B resistance marker from Escherichia coli.
    7閵嗕笚unctional elements in the promoter region of the nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase

    pAN7-1-Plasmid

    pAN7- 1 Plasmid Sequence

    LOCUS       Z32698                  6756 bp    DNA     linear   SYN 14-NOV-2006
    DEFINITION  Expression vector (PAN7-1) DNA, 6756bp.
    ACCESSION   Z32698
    VERSION     Z32698.1  GI:475166
    KEYWORDS    hygromycin b phosphotransferase.
    SOURCE      synthetic construct
      ORGANISM  synthetic construct
                other sequences; artificial sequences.
    REFERENCE   1
      AUTHORS   Mullaney,E.J., Hamer,J.E., Roberti,K.A., Yelton,M.M. and
                Timberlake,W.E.
      TITLE     Primary structure of the trpC gene from nidulans
      JOURNAL   Mol. Gen. Genet. 199 (1), 37-45 (1985)
       PUBMED   3158796
      REMARK    sites .
    REFERENCE   2
      AUTHORS   Punt,P.J., Oliver,R.P., Dingemanse,M.A., Pouwels,P.H. and van den
                Hondel,C.A.
      TITLE     Transformation of based on the hygromycin B resistance
                marker from Escherichia coli
      JOURNAL   Gene 56 (1), 117-124 (1987)
       PUBMED   2824287
      REMARK    (sites)
    REFERENCE   3  (bases 999 to 2572)
      AUTHORS   Punt,P.J., Dingemanse,M.A., Kuyvenhoven,A., Soede,R.D.,
                Pouwels,P.H. and van den Hondel,C.A.
      TITLE     Functional elements in the promoter region of the
                nidulans gpdA gene encoding glyceraldehyde-3-phosphate
                dehydrogenase
      JOURNAL   Gene 93 (1), 101-109 (1990)
       PUBMED   2121607
    REFERENCE   4  (bases 1 to 6756)
      AUTHORS   Punt,P.
      TITLE     Direct Submission
      JOURNAL   Submitted (22-APR-1994) Punt P., TNO Nutrition and Food Research
                Institute, Lange Kleiweg 139, Rijkswik, The Netherlands
    FEATURES             Location/Qualifiers
         source          1..6756
                         /organism="synthetic construct"
                         /mol_type="other DNA"
                         /db_xref="taxon:32630"
         misc_feature    1..6756
                         /product="fungal transformation vector pAN7-1"
                         /standard_name="HPH"
                         /experiment="experimental evidence, no additional details
                         recorded"
                         /citation=[2]
                         /phenotype="gene product confers resistance to hygromycin
                         B"
         regulatory      1..2129
                         /regulatory_class="promoter"
                         /experiment="experimental evidence, no additional details
                         recorded"
                         /citation=[2]
                         /citation=[3]
                         /function="promoter region of the A.nidulans gpdA gene"
         gene            2130
                         /gene="gpdA"
         misc_feature    2130
                         /gene="gpdA"
                         /experiment="experimental evidence, no additional details
                         recorded"
                         /citation=[3]
                         /function="major transcription start site"
         intron          2178..2293
                         /experiment="experimental evidence, no additional details
                         recorded"
                         /citation=[3]
                         /number=1
         gene            2302..3321
                         /gene="HPH"
         CDS             2302..3321
                         /gene="HPH"
                         /experiment="experimental evidence, no additional details
                         recorded"
                         /note="gene-product confers resistance to hygromycin B"
                         /citation=[2]
                         /citation=[3]
                         /codon_start=1
                         /transl_table=11
                         /product="hygromycin B phosphotransferase"
                         /protein_id="CAA83647.1"
                         /db_xref="GI:475167"
                         /translation="MPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                         VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                         ETELPAVLQPVAEAAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                         QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEA
                         MFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSL
                         VDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTR
                         PRAKE"
         regulatory      3339..4108
                         /regulatory_class="terminator"
                         /standard_name="terminator region from A.nidulans trpC
                         gene (containing CDS)"
                         /experiment="experimental evidence, no additional details
                         recorded"
                         /note="this region contains the 3'end of the trpC
                         transcript including 50 codons. two major transcription
                         ends have been identified"

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    Structural basis for the interaction of the chaperone Cbp3 with newly synthesized cytochrome b during mitochondrial respiratory chain assembly Mama Ndi1, Geoffrey Masuyer1, Hannah Dawitz1, Andreas Carlstr?m1, Mirco Michel1, Arne Elofsson2, Mikaela Rapp1, P?l Stenmark3 and Martin Ott3* First Published on Journal of biological chemistry September 19, 2019 doi: 10.1074/jbc.RA119.010483 jbc.RA119.010483.
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