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- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pN3
- 库存：
100
- 供应商：
LSMBIO
- 规格：
2ug
pN3
Search name
pN3,Plasmid pN3,pN3 vector
pN3 Information
Promoter: CMV
Replicator: pUC ori, F1 ori
Terminator: SV40 poly (A) signal
Plasmid classification: mammalian cells, fluorescent protein reporting carriers
Plasmid size: 3996bp
Prokaryotic resistance: Kan
Screening markers: Neo
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, instantaneous expression
5'sequencing primers: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'sequencing primers: primers designed according to sequence
pN3 Description
PN3 is a mammalian cell, fluorescent protein reporter vector. Carrier size 3996bp, Neo (G418) can be used for screening.
pN3 Sequence
LOCUS Exported 3996 bp ds-DNA circular SYN 31-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3996)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, August 31, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..3996
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
polyA_signal 784..905
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(912..1367)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1394..1498
/gene="bla"
/note="AmpR promoter"
promoter 1500..1857
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1708..1843
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 1892..2686
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 2918..2965
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3294..3882
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
721 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
781 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
841 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
901 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
961 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
1021 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
1081 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
1141 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
1201 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
1261 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
1321 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
1381 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
1441 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
1501 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
1561 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
1621 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
1681 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
1741 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
1801 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
1861 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
1921 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
1981 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
2041 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
2101 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
2161 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
2221 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
2281 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
2341 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
2401 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
2461 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
2521 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
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文献和实验该产品被引用文献
Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation
Author：YangZhang1YiXu1WenyunLu23Jonathan M.Ghergurovich24LiliGuo56Ian A.Blair5Joshua D.Rabinowitz23XiaoluYang17
Received 22 January 2020, Revised 4 August 2020, Accepted 30 September 2020, Available online 6 November 2020.
Published: November 6, 2020
Cell Metabolism
Available online 6 November 2020 In Press
doi: doi.org/10.1016/j.cmet.2020.10.002
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pN3
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