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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pLKO.5-TRC2-Puro
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pLKO.5-TRC2-Puro
Catalog No. PVT11109
Packing 2ug
pLKO.5-TRC2-Puro Informaiton
Promoter: U6
Replicon: pUC ori, F1 ori
Plasmid classification: virus series, lentivirus interference vector
Plasmid size: 7484bp
Prokaryotic resistance: Amp
Selection marker: Puro
Clone strain: Stbl3
Culture conditions: 37 C, aerobic LB
Expression host: lactation cells
Induction mode: no need to induce, transient expression.
5'sequencing primers: U6:ATGGACTATCATATGCTTACCGTA
Primers for 3'sequencing: primers designed based on sequences
pLKO.5-TRC2-Puro Description
The MISSION TRC2 Control Vector pLKO-puro is a lentivirus plasmid vector. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the TRC2 MISSION shRNA library clones. This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones.
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文献和实验pLKO.1-EGFP 病毒质粒 Amp pLKO.1 病毒质粒 pLKO.1-TRC 病毒质粒 http://www.addgene.org/10878/ pLKO.1-Puro
一、材料和试剂 1. Hairpin-pLKO.1vector(OpenBiosystems) 2. Packagingvectors:第二代包装质粒包含gag,pol,和rev基因(pCMV-dR8.91或pCMV-dR8.74psPAX2)和包被质粒(pMD2.G)(Addgene) 3. 脂质体2000(Invitrogen) 4.OptiMEM无血清培养基(Invitrogen) 5. 293T包装细胞(ATCC)
关键词: 副溶血性弧菌 直接溶血毒素基因原核表达载体 构建 副溶血性弧菌(Vibrio parahaemolyticus,Vp)是一种能引起食物中毒和旅游者腹泻的嗜盐性细菌。直接溶血毒素(thermostable direct hemolysin,TDH)是其主要的致病因子。本研究通过构建tdh基因原核表达载体,并实现表达,以获得与天然抗原结构接近的重组抗原。 构建重组质粒tdh/Trc99A经酶切及PCR
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