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- PVT10391
- 2025年08月31日
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- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pCDEF-HA-Ub-K63R
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pCDEF-HA-Ub-K63R
Catalog No. PVT10391
Packing 2ug
pCDEF-HA-Ub-K63R Information
Function ORF expression plasmid
Resistance Amp
Strain DH5alpha
Culture temperature 37degrees centigrade
pCDEF-HA-Ub-K63R Description
1, pCDEF-HA-Ub -K63R is the expression plasmid of mammalian cells, and the prokaryotic resistance is Amp (ampicillin). It is mainly used to express Ub gene in mammalian cells and its related research.
2. The product contains plasmid protectant developed by our company, which is very beneficial to long-term preservation, but it can not be used directly for enzyme digestion.
3, please take 1ul plasmids in time to transfer to the DH5 alpha highly highly sensitive cells supplied by our department, pick up the liquid LB for the single colony 37鈩? and extract the plasmids and keep the glycerol bacteria, and keep it at -80鈩?for a long time.
pCDEF-HA-Ub-K63R
1, there are 7 lysine in the Ub gene, which are sixth, 11, 27, 29, 33, 48, 63. The pCDEF-HA-Ub-K63R plasmids only turn sixty-third lysine into arginine and retain the remaining six lysine, which can be used to study which site of the Ub chain.
2. The pCDEF vector is constructed from pCDNA1 and pEF-BOS, and the CMV promoter is replaced by EF1 alpha promoter.
3, the original sequence of the Ub gene:
ATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGA
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文献和实验因子,使TH2型反应向TH1型反应转变。因此称之为“免疫刺激DNA序列”(ISS)。ISS的精确序列及在质粒中所处位置对DNA免疫都有影响。含CpG的免疫刺激序列不仅能增强DNA的免疫,还能直接增强蛋白质的免疫。1998年,Lee等对CpGDNA的佐剂效应作深入的研究可证实这点：当用95ug的质粒载体pCIN(含ISS)和5ug表达流感病毒凝集素(HA)的质粒pCIN HA共注射小鼠后,得到的CTL效应和T细胞增殖率比单注射5ug或100ug的pCINHA高,特异性IgG与注 射100ug
刺激DNA序列”(ISS)。ISS的精确序列及在质粒中所处位置对DNA免疫都有影响。含CpG的免疫刺激序列不仅能增强DNA的免疫,还能直接增强蛋白质的免疫。1998年,Lee等对CpGDNA的佐剂效应作深入的研究可证实这点：当用95ug的质粒载体pCIN(含ISS)和5ug表达流感病毒凝集素(HA)的质粒pCIN HA共注射小鼠后,得到的CTL效应和T细胞增殖率比单注射5ug或100ug的pCINHA高,特异性IgG与注 射100ug的pCINHA相当。但IgG2a/IgG1较低,抗体谱仍以IgG
(2 µg), and 1 µg of purified Ub‐AMC and HA‐tagged Ub‐AMC (HA‐Ub‐AMC). Free Ub (1 µg) was loaded for comparison. (C ) SDS‐PAGE analysis and CBB staining of purified USP14 WT and CA. A known amount of BSA was loaded for comparison. RP2 CP indicates doubly
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