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- PVTB50014-2b
- 2025年07月16日
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- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pCMV-Myc-p47 E218A
- 库存：
100
- 供应商：
LSMBIO
- 规格：
2ug
pCMV-Myc-p47 E218A Plasmid
Catalog No. PVTB50014-2b
Vector Backbone pCMV-Myc
Backbone manufacturer Clontech
Vector type Mammalian Expression
Backbone size w/o insert 3800bp
Cloning site 5 EcoR I
Site destroyed during cloning NO
Cloning site 3 Xho I
Bacterial resistance Ampicillin
Growth strain DH5alpha
Growth temperature 37 Centigrade
High or low copy High Copy
Selectable markers None
Background Mammalian Expression vector of Mus musculus neutrophil cytosolic factor 1(one point mutation E218A) with N terminal Myc tag
Gene Information
Alternative Gene Name Ncf1; NOXO2; Ncf-1; NCF-47K; p47phox; p47-phox; p47
Insert Gene name p47(E218A)
Insert size 1173bp
Accessions AAF90134.1
Tag Myc(N-term)
Species Mus musculus (house mouse)
Gene ID 17969
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文献和实验Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌
Donis-Keller Lab Manual, Department of Genetics in Washington University School of MedicinePurpose: To utilize competent E. coli bacteria to replicate a specific DNA fragment. Three methods are presented transformation of intact plasmids/cosmids
碱裂解法制备大肠杆菌质粒（Alkaline Lysis Plasmid Preparation from E.
相关专题 This protocol yields plasmid DNA that is suitable for restriction digests and cloning purposes. This preparation method works well on all E. coli strains and also exponentially growing Rhizobium meliloti cultures
/actions/archive/post/4411006_0.html pCANTAB5E及PHEN1的质粒载体图谱 http://www.dxy.cn/bbs/thread/8547369 真核表达载体ppic9和ppic9k载体的图谱：http://www.dxy.cn/bbs/actions/archive/post/162977_0.html 质粒载体PJLA503 的图谱 http://www.dxy.cn/bbs/actions/archive/post/1833996_0.html
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