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- LSM BIO
- 进口
- PVTB80035-2a
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
p3*Flag-CMV-10-Npro
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
p3*Flag-CMV-10-Npro Plasmid?
Catalog No. PVTB80035-2a
Vector Backbone p3*Flag-CMV-10
Backbone manufacturer Sigma
Vector type Mammalian Expression
Backbone size w/o insert 6400bp
Cloning site 5 EcoR I
Site destroyed during cloning NO
Cloning site 3 BamH I
Bacterial resistance Ampicillin
Growth strain Top10
Growth temperature 37 Centigrade
High or low copy High Copy
Selectable markers Neomycin
Background N/A
Gene Information
Alternative Gene Name N/A
Insert Gene name Npro
Insert size 504bp
Accessions AF333000
Tag 3*Flag(N-term)
Species Classical swine fever virus
Gene ID ?
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文献和实验, mix the solution gently, but thoroughly, immediately after addition of Buffer P3. Mix by inverting the tube 4-6 times. 4. Centrifuge at maximum speed in a microcentrifuge for 10 min. Remove supernatant promptly. 用微量离心器在最大速度下离心10 min，迅速移出
Plasmid Mini Purification Protocol（质粒纯化）
of Buffer P3. Mix by inverting the tube 4-6 times.4. Centrifuge at maximum speed in a microcentrifuge for 10 min. Remove supernatant promptly. 用微量离心器在最大速度下离心10 min，迅速移出上清。Before loading the centrifuge, the sample should be mixed again. Centrifugation
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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