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- LSM BIO
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- PVTB80033-2a
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
p3*Flag-CNS5A
- 库存：
10
- 供应商：
LSMBIO
- 规格：
质粒干粉
p3*Flag-CNS5A Plasmid?
Catalog No. PVTB80033-2a
Vector Backbone p3*Flag-CMV-10
Backbone manufacturer Sigma
Vector type Mammalian Expression
Backbone size w/o insert 6400bp
Cloning site 5 EcoR I
Site destroyed during cloning NO
Cloning site 3 BamH I
Bacterial resistance Ampicillin
Growth strain Top10
Growth temperature 37 Centigrade
High or low copy High Copy
Selectable markers Neomycin
Background N/A
Gene Information
Alternative Gene Name HCLV
Insert Gene name CNS5A
Insert size 1491bp
Accessions AF531433
Tag 3*Flag(N-term)
Species Classical swine fever virus strain
Gene ID ?
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文献和实验This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
Plasmid Mini Purification Protocol（质粒纯化）
-through and save for an analytical gel sample 2.7. Wash the QIAGEN-tip 20 with 4×1 ml Buffer QC. 用4×1 ml缓冲液QC对QIAGEN-tip 20尖管进行清洗。 Allow Buffer QC to move through the QIAGEN-tip by gravity flow. The first 2 ml is sufficient to remove all contaminants in the majority of plasmid DNA
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